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产品描述：人细胞周期蛋白依赖性激酶抑制剂2A，亚型1/2/3(CDKN2A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CDKN2A含量。CDKN2A 是重要的肿瘤抑制基因，在细胞周期调控中发挥关键作用。其编码的 p16 蛋白可抑制 CDK4/6 激酶活性，阻滞细胞从 G1 期进入 S 期。研究发现该靶点失活与多种肿瘤发生发展相关，对其机制的深入探究有助于开发新型抗癌疗法。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研中探究肿瘤发生机制、细胞周期调控网络、药物干预效果评估等方向，为体外实验提供可靠的蛋白定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CDKN2A ELISA Kit; CDKN2 ELISA Kit; MTS1 ELISA Kit; Cyclin-dependent kinase inhibitor 2A ELISA Kit; Cyclin-dependent kinase 4 inhibitor A ELISA Kit; CDK4I ELISA Kit; Multiple tumor suppressor 1 ELISA Kit; MTS-1 ELISA Kit; p16-INK4a ELISA Kit; p16-INK4 ELISA Kit; p16INK4A ELISA Kit
缩写：CDKN2A
Uniprot No.：P42771
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.69 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CDKN2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 106 | |||||
| Range % | 101-112 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 92-99 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 86-96 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-99 | ||||||
回收率：
| The recovery of human CDKN2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-107 | |||||
| EDTA plasma (n=4) | 93 | 85-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected12002.3552.4432.3992.3066001.8061.7841.7951.7023001.3331.2301.2821.1891500.8410.9060.8740.781750.4620.4600.4610.36837.50.2740.2900.2820.18918.750.1630.1600.1620.06900.0950.0900.093 | |||||||
货期：3-5 working days
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