首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人可溶性CD38(sCD38)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD38含量。CD38是一种跨膜糖蛋白，广泛表达于多种免疫细胞。其在调节细胞黏附、迁移、信号转导等方面有重要作用。研究机制上，它可作为靶点被单克隆抗体等药物靶向结合，阻断相关信号通路，以发挥抗肿瘤、调节免疫等潜在治疗功效 。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于基础科研中sCD38表达水平的动态监测、疾病模型机制研究以及生物标志物筛选等应用场景；支持科研人员高效开展免疫学、肿瘤学及细胞生物学领域的定量分析工作。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD38; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD antigen CD38
缩写：CD38
Uniprot No.：P28907
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD38 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:10 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human sCD38 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 82-96 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.2722.3722.3222.155301.7791.8591.8191.652151.2091.2291.2191.0527.50.7240.7400.7320.5653.750.4290.4390.4340.2671.870.3390.3470.3430.1760.90.2350.2390.2370.07000.1660.1680.167 | ||||||
货期：3-5 working days
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