首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CC趋化因子受体2(CCR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）样本中的CCR2含量。CCR2是一种重要的趋化因子受体，参与调节单核细胞和巨噬细胞的迁移，以及T细胞炎症细胞因子的产生。其研究机制涉及CCL2/CCR2信号通路，该通路在脊髓损伤、肿瘤免疫治疗等领域具有潜在的治疗价值。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于科研领域中对CCR2表达水平的精准分析；在神经炎症研究中可评估脑脊液内CCR2的动态变化；在心血管疾病模型中量化血浆及组织匀浆的趋化因子受体表达差异；为探索炎症相关疾病的分子机制、药物靶点筛选及病理模型验证提供了可靠工具，广泛应用于基础医学、免疫学及药理学研究领域，为科研人员解析CCR2在疾病发生发展中的调控网络提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCR2; CMKBR2; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R; CD antigen CD192
缩写：CCR2
Uniprot No.：P41597
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human CCR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-106 | |||||
回收率：
| The recovery of Human CCR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-106 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.7252.5292.6272.4457502.0461.9882.0171.8353751.4541.4411.4481.266187.50.9220.8850.9040.72293.750.5870.5840.5860.40446.880.4110.4150.4130.23123.440.2870.2810.2840.10200.1820.1810.182 | ||||||
货期：3-5 working days
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