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产品描述：人G1/S特异性细胞周期蛋白E1(CCNE1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CCNE1含量。CCNE1 是细胞周期蛋白 E1 编码基因，在细胞周期调控中至关重要。其异常表达与多种肿瘤的发生、发展相关。研究机制主要聚焦于其通过与 CDK2 形成复合物，推动细胞从 G1 期进入 S 期，影响细胞增殖、迁移和侵袭能力。试剂盒检测范围为25 pg/mL-1600 pg/mL，本产品广泛应用于基础科研领域，包括细胞周期动力学研究、肿瘤生物学机制探索、抗癌药物筛选模型构建以及疾病相关分子标志物的体外定量分析，为揭示CCNE1在细胞增殖、基因组稳定性及癌症进展中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCNE ELISA Kit; Ccne1 ELISA Kit; CCNE1_HUMAN ELISA Kit; cyclin E variant ex5del ELISA Kit; cyclin E variant ex7del ELISA Kit; Cyclin E1 ELISA Kit; Cyclin Es ELISA Kit; Cyclin Et ELISA Kit; CyclinE ELISA Kit; G1/S specific cyclin E ELISA Kit; G1/S-specific cyclin-E1 ELISA Kit
缩写：CCNE1
Uniprot No.：P24864
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCNE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 100 | |||||
| Range % | 95-104 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:8 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
回收率：
| The recovery of human CCNE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 89-97 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16001.9211.8371.8791.7418001.2941.2281.2611.1234000.8190.7830.8010.6632000.5430.5190.5310.3931000.3620.3760.3690.231500.2800.3010.2910.153250.2070.1940.2010.06300.1410.1350.138 | |||||||
货期：3-5 working days
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