首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠正常T细胞表达和分泌因子/趋化因子5(RANTES/CCL5)酶联免疫试剂盒）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的RANTES/CCL5含量。RANTES/CCL5是一种趋化因子，在免疫调节、炎症反应等方面有重要背景。其研究机制涉及多种细胞，能吸引特定免疫细胞到炎症部位，参与免疫应答。它与多种疾病如感染、自身免疫病相关，对它的研究有助于深入了解疾病发病机理与治疗。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外实验中对大鼠疾病模型（如关节炎、神经炎症或病毒感染模型）中RANTES动态表达的定量分析，也可用于药物研发过程中评价免疫调节剂或抗炎药物对趋化因子通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
缩写：RANTES/CCL5
Uniprot No.：P50231
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 99 | |||||
| Range % | 94-105 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 97-106 | ||||||
回收率：
| The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-92 | |||||
| EDTA plasma (n=4) | 92 | 86-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.6842.7992.7422.63352.1782.3492.2642.1552.51.4441.4891.4671.3581.250.7830.7950.7890.6800.6250.3760.3920.3840.2750.3120.2580.2640.2610.1520.1560.2010.2080.2050.09600.1080.1090.109 | |||||||
货期：3-5 working days
×
