首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠巨噬细胞炎症蛋白1β(MIP-1β/CCL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL4含量。CCL4即趋化因子配体4，在免疫调节等生理过程中起重要作用。其背景与免疫细胞的招募和活化密切相关。研究机制上，CCL4通过与相应受体结合，引导免疫细胞迁移、调控免疫反应，在炎症、肿瘤等疾病病理进程中有相关研究。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于炎症相关动物模型构建、免疫调控机制解析、药理学研究中生物标志物动态监测等科研场景，为研究者探索巨噬细胞活化、趋化因子网络交互作用等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl4 ELISA Kit; Mip1b ELISA Kit; Scya4C-C motif chemokine 4 ELISA Kit; Macrophage inflammatory protein 1-beta ELISA Kit; MIP-1-beta ELISA Kit; Small-inducible cytokine A4 ELISA Kit
缩写：CCL4
Uniprot No.：P50230
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 106 | ||||
| Range % | 101-112 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rat MIP-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5642.4762.5202.3751002.1742.0142.0941.949501.4181.3721.3951.250250.9170.9730.9450.80012.50.5550.5890.5720.4276.250.3940.4170.4060.2613.120.2890.2730.2810.13600.1480.1410.145 | ||||||
货期：3-5 working days
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