Recombinant Human PHB2 protein

中文名： 抗增殖蛋白2(PHB2)重组蛋白
别名： PHB2;BAP;REA;Prohibitin-2

货号: PA1000-2378

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	PHB2
Uniprot No	Q99623
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-299aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMGSMAQNLKDLAGRLPAGPRGMGTALKLLL GAGAVAYGVRESVFTVEGGHRAIFFNRIGGVQQDTILAEGLHFRIPWFQY PIIYDIRARPRKISSPTGSKDLQMVNISLRVLSRPNAQELPSMYQRLGLD YEERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSL ILDDVAITELSFSREYTAAVEAKQVAQQEAQRAQFLVEKAKQEQRQKIVQ AEGEAEAAKMLGEALSKNPGYIKLRKIRAAQNISKTIATSQNRIYLTADN LVLNLQDESFTRGSDSLIKGKK
预测分子量	36 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PHB2重组蛋白的3篇参考文献及摘要概括：

1. **文献名称**: "Recombinant Human Prohibitin 2 (PHB2) Protein: Expression, Purification, and Structural Characterization"

**作者**: Zhang Y, et al.

**摘要**: 本研究报道了利用大肠杆菌表达系统成功表达并纯化重组人源PHB2蛋白，通过圆二色谱和X射线晶体学分析其二级结构及三维构象，证实重组PHB2具有与天然蛋白相似的折叠特性，为后续功能研究奠定基础。

2. **文献名称**: "PHB2 Mediates Mitochondrial Autophagy via Interaction with LC3 in Mammalian Cells"

**作者**: Wei Y, et al.

**摘要**: 该研究通过体外表达纯化的重组PHB2蛋白，结合免疫共沉淀实验，揭示PHB2通过直接结合自噬标志蛋白LC3.调控受损线粒体的选择性清除(线粒体自噬)，为神经退行性疾病机制提供了新见解。

3. **文献名称**: "Prohibitin 2 Regulates Apoptosis through Interaction with ANXA7 in Cancer Cells"

**作者**: Li H, et al.

**摘要**: 研究利用重组PHB2蛋白进行蛋白质相互作用分析，发现PHB2与膜联蛋白ANXA7结合并抑制其促凋亡活性，揭示了PHB2在肿瘤细胞凋亡抵抗中的分子机制，为靶向PHB2的抗癌策略提供依据。

注：以上文献为示例，实际引用需核对真实文献信息。

背景信息

**Background of PHB2 Recombinant Protein**

Prohibitin-2 (PHB2), a member of the prohibitins family, is a highly conserved protein ubiquitously expressed in eukaryotic cells. Initially identified as an antiproliferative protein, PHB2 shares structural homology with PHB1. forming a heterodimeric complex in the mitochondrial inner membrane. This complex plays a critical role in maintaining mitochondrial integrity, including cristae morphogenesis, oxidative phosphorylation, and reactive oxygen species (ROS) regulation. Beyond its mitochondrial role, PHB2 localizes to the nucleus, where it participates in transcriptional regulation, chromatin remodeling, and cell cycle control.

Recombinant PHB2 protein is engineered through molecular cloning techniques, often expressed in bacterial or mammalian systems, followed by purification to ensure high specificity and activity. Its production enables researchers to study PHB2's molecular interactions, post-translational modifications, and functional mechanisms in vitro. Studies using recombinant PHB2 have elucidated its involvement in cellular stress responses, apoptosis, and aging. For instance, PHB2 interacts with proteins like PARL and Drp1. modulating mitochondrial dynamics and mitophagy.

Emerging evidence links PHB2 dysregulation to pathologies, including cancer, neurodegeneration, and metabolic disorders. In cancer, PHB2 exhibits dual roles, acting as a tumor suppressor or promoter depending on cellular context. Its nuclear functions also implicate PHB2 in epigenetic regulation, influencing gene expression patterns associated with disease progression. Recombinant PHB2 serves as a valuable tool for drug screening, structural studies, and therapeutic development targeting mitochondrial or nuclear pathways.

Overall, PHB2's multifunctional nature underscores its biological significance, with recombinant protein technology accelerating mechanistic insights and translational applications.