首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胆囊收缩素/缩胆囊素八肽(CCK-8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CCK-8含量。CCK-8即细胞计数试剂盒-8，是常用的检测细胞增殖和细胞毒性的试剂。其背景源于对高效便捷细胞活性检测方法的需求。研究机制是基于WST-8在电子载体作用下被细胞线粒体中的脱氢酶还原为具有高度水溶性的甲臜产物，通过测定吸光度反映细胞活性。试剂盒检测范围为0.57 ng/mL-10 ng/mL，适用于胃肠动力学研究、神经内分泌调控机制探索、代谢性疾病动物模型评价等科研领域。可满足基础研究中对CCK - 8动态监测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CCK-8
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.57 ng/mL-10 ng/mL
灵敏度：0.357 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCK-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human CCK-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-108 | ||||
| EDTA plasma (n=4) | 95 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average0.570.0480.0510.0501.140.1620.1530.1582.290.3760.3630.3705.710.8380.8420.840101.6551.6671.661 | ||||||
货期：3-5 working days
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