首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCK含量。CCK即胆囊收缩素，是一种广泛分布于胃肠道和中枢神经系统的脑肠肽。其可调节胃肠运动、胆囊收缩等。在研究机制上，CCK通过与特异性受体结合发挥作用，参与多种生理和病理过程，对其研究有助于深入理解消化等生理功能及相关疾病机制。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探究消化系统功能、营养代谢调控、神经 - 胃肠轴互作等研究领域；科研人员可通过定量分析不同生理或实验条件下（如摄食行为干预、肠道疾病模型构建等）大鼠体内CCK的表达水平变化，为胃肠生理学、神经内分泌学等基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CckCholecystokinin ELISA kit; CCK) [Cleaved into: Cholecystokinin-39 ELISA kit; CCK39); Cholecystokinin-33 ELISA kit; CCK33); Cholecystokinin-22 ELISA kit; CCK22); Cholecystokinin-12 ELISA kit; CCK12); Cholecystokinin-8 ELISA kit; CCK8)] ELISA kit
缩写：CCK
Uniprot No.：P01355
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of rat CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 96-108 | ||||
| EDTA plasma (n=4) | 90 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.4492.3512.4002.209 5001.7831.6921.7381.547 2501.2121.1821.1971.006 1250.7890.7390.7640.573 62.50.5490.5960.5730.382 31.20.4090.4200.4150.224 15.60.2670.2780.2730.082 00.1850.1970.191 | ||||||
货期：3-5 working days
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