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Human cholecystokinin,CCK ELISA Kit

  • 中文名: 人胆囊收缩素/肠促胰酶肽(CCK)ELISA KIT
货号: IPD-E08116h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CCK含量。CCK即胆囊收缩素,在生理上可调节胆囊收缩、胃肠运动等。研究机制方面,它通过与相关受体结合发挥作用,在神经系统和消化系统中参与多种生理功能调节,其信号通路的研究对理解神经胃肠病等疾病发病机制有重要意义。试剂盒检测范围为56 pg/mL-800 pg/mL,该产品可应用于胃肠功能研究、摄食行为神经机制探索、代谢综合征模型评估等科研领域,特别适合需要精确测定生物体液或组织样本中CCK动态变化的实验设计,其宽检测范围可满足基础研究中正常生理水平到病理状态下的浓度检测需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    (1-49)-CCK58 ELISA Kit; CCK ELISA Kit; CCK12 ELISA Kit; CCK18 ELISA Kit; CCK25 ELISA Kit; CCK33 ELISA Kit; CCK39 ELISA Kit; CCK5 ELISA Kit; CCK58 ELISA Kit; CCK7 ELISA Kit; CCK8 ELISA Kit; CCKN_HUMAN ELISA Kit; Cholecystokinin-5 ELISA Kit
  • 缩写:
    CCK
  • Uniprot No.:
    P06307
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    56 pg/mL-800 pg/mL
  • 灵敏度:
    20 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    80-98


    1:2

    Average %

    95


    Range %

    91-105


    1:4

    Average %

    87


    Range %

    82-94


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率:

    The recovery of human CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    93

    90-100


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    800

    2.453

    2.504

    2.478

    2.478


    400

    1.275

    1.287

    1.281

    1.281


    280

    0.756

    0.748

    0.752

    0.752


    144

    0.395

    0.410

    0.402

    0.402


    56

    0.200

    0.205

    0.203

    0.203


    0

    0.112

    0.113

    0.113

    0.113



  • 货期:
    3-5 working days

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