首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胱硫醚β-合酶(CBS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CBS含量。人胱硫醚β-合酶（CBS）是转硫途径中的关键酶，参与同型半胱氨酸（Hcy）的代谢，其活性与心血管疾病风险相关。研究显示，CBS在Hcy转化为胱硫醚的转硫酸路径中起重要作用，其表达上调与多种疾病如唐氏综合症和癌症有关。抑制CBS可能对治疗这些疾病有益。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于研究CBS在细胞代谢调控、氧化应激响应及疾病模型中的表达变化，也可用于评估药物或基因干预对CBS活性的影响，为探究相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI047524 ELISA Kit; AI303044 ELISA Kit; Beta thionase ELISA Kit; Beta-thionase ELISA Kit; Cbs ELISA Kit; Cbs cystathionine beta-synthase ELISA Kit; CBS_HUMAN ELISA Kit; Cystathionine beta synthase ELISA Kit; Cystathionine beta-synthase ELISA Kit; EC 4.2.1.22 ELISA Kit; HIP 4 ELISA Kit; HIP4 ELISA Kit; Methylcysteine synthase ELISA Kit; MGC18856 ELISA Kit; MGC18895 ELISA Kit; MGC37300 ELISA Kit; OTTHUMP00000109416 ELISA Kit; OTTHUMP00000109418 ELISA Kit; Serine sulfhydrase ELISA Kit
缩写：CBS
Uniprot No.：P35520
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CBS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 102-110 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 90-101 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 86-96 | ||||||
回收率：
| The recovery of human CBS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 98-106 | |||||
| EDTA plasma (n=4) | 94 | 91-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected80002.1442.0332.0891.97840001.3881.4111.4001.28920000.9320.9890.9610.85010000.6550.6360.6460.5355000.4450.4240.4350.3242500.2660.2680.2670.1561250.2120.2210.2170.10600.1100.1120.111 . | |||||||
货期：3-5 working days
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