首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人羰基还原酶[NADPH] 1 (CBR1/CBR/CRN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CBR1含量。CBR1即大麻素受体 1，广泛分布于中枢神经系统等部位。它与内源性大麻素系统密切相关，参与调控食欲、记忆、情绪等生理过程。研究机制方面，聚焦其信号通路、与配体相互作用等，探索通过调节CBR1治疗相关疾病的可能。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域中对CBR1表达调控的探索，如药物代谢动力学研究、肿瘤耐药机制分析、氧化应激相关疾病模型构建等基础研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：15 hydroxyprostaglandin dehydrogenase [NADP+] ELISA Kit; 15-hydroxyprostaglandin dehydrogenase [NADP+] ELISA Kit; Carbonyl reductase [NADPH] 1 ELISA Kit; Carbonyl Reductase 1 ELISA Kit; CBR 1 ELISA Kit; CBR1 ELISA Kit; CBR1_HUMAN ELISA Kit; CRN ELISA Kit; NADPH dependent carbonyl reductase 1 ELISA Kit; NADPH-dependent carbonyl reductase 1 ELISA Kit; Prostaglandin 9 ketoreductase ELISA Kit; Prostaglandin 9-ketoreductase ELISA Kit; Prostaglandin E(2) 9 reductase ELISA Kit; Prostaglandin-E(2) 9-reductase ELISA Kit; SDR21C1 ELISA Kit
缩写：CBR1
Uniprot No.：P16152
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CBR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 98-107 | ||||||
回收率：
| The recovery of human CBR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 92-100 | |||||
| EDTA plasma (n=4) | 89 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.3192.2142.2672.14210001.3941.4521.4231.2985000.8250.8700.8480.7232500.4710.4870.4790.3541250.3080.2950.3020.17762.50.2470.2390.2430.11831.250.1530.1610.1570.03200.1220.1280.125 | |||||||
货期：3-5 working days
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