首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人窖蛋白Caveolin1(Cav-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CAV1含量。CAV1 即 Caveolin-1，是细胞膜穴样凹陷的主要结构蛋白。它参与细胞内吞、胆固醇转运等多种生理过程。在疾病研究中，其表达变化与肿瘤的发生、发展、转移等密切相关，还涉及心血管疾病等的病理机制，是潜在的治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可将其应用于基础医学研究中的细胞模型、动物疾病模型或临床前样本分析，通过检测Cav - 1的动态表达水平，探索其在细胞功能调控、疾病分子机制中的潜在作用，尤其适用于肿瘤微环境研究、代谢性疾病相关信号通路分析及组织纤维化分子标志物筛选等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BSCL3 ELISA Kit; CAV ELISA Kit; CAV1 ELISA Kit; CAV1_HUMAN ELISA Kit; caveolae protein, 22 kD ELISA Kit; caveolin 1 alpha isoform ELISA Kit; caveolin 1 beta isoform ELISA Kit; Caveolin 1 caveolae protein 22kDa ELISA Kit; Caveolin-1 ELISA Kit; Caveolin1 ELISA Kit; cell growth-inhibiting protein 32 ELISA Kit; CGL3 ELISA Kit; LCCNS ELISA Kit; MSTP085 ELISA Kit; OTTHUMP00000025031 ELISA Kit; PPH3 ELISA Kit; VIP 21 ELISA Kit; VIP21 ELISA Kit
缩写：CAV1
Uniprot No.：Q03135
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Cav-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human Cav-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 95-107 | ||||
| EDTA plasma (n=4) | 95 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5012.5522.5272.42410001.8632.0021.9331.8305001.2541.2891.2721.1692500.8210.8330.8270.7241250.4980.5350.5170.41462.50.3280.3460.3370.23431.250.2060.2010.2040.10100.1040.1010.103 | ||||||
货期：3-5 working days
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