首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠过氧化氢酶(CAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CAT含量。试剂盒检测范围为93.75 mIU/mL-6000 mIU/mL，适用于氧化应激机制研究、药物毒性评估及炎症反应模型等科研领域，为心血管疾病、神经退行性疾病等动物模型中的抗氧化系统功能研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cat ELISA kit; Cas1Catalase ELISA kit; EC 1.11.1.6 ELISA kit
缩写：CAT
Uniprot No.：P04762
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates
检测范围：93.75 mIU/mL-6000 mIU/mL
灵敏度：23.43 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 90-97 | |||||
| 1:20 | Average % | 105 | ||||
| Range % | 98-109 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of rat CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-94 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 60002.7032.6022.6532.537 30001.9621.8611.9121.796 15001.2181.1971.2081.092 7500.7340.7230.7290.613 3750.3870.3980.3930.277 187.50.2970.2860.2920.176 93.750.1880.1840.1860.070 00.1150.1170.116 | ||||||
货期：3-5 working days
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