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产品描述：人过氧化氢酶(CAT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CAT含量。人过氧化氢酶（CAT）是一种广泛存在于生物体中的抗氧化酶，其主要功能是催化过氧化氢分解成氧气和水，从而保护细胞免受氧化应激损伤。研究显示，CAT通过基因工程改造可提高其活性，在食品、医药等领域有广泛应用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，广泛应用于基础医学研究中氧化应激机制的探索，如神经退行性疾病、代谢综合征、心血管疾病等病理模型中的抗氧化能力评估，也可用于天然产物或合成化合物清除自由基效果的体外验证本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cas1 ELISA Kit; CAT ELISA Kit; CATA_HUMAN ELISA Kit; Catalase ELISA Kit; Cs1 ELISA Kit; MGC138422 ELISA Kit; MGC138424 ELISA Kit
缩写：CAT
Uniprot No.：P04040
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4202.3332.3772.2825002.1082.0472.0041.9092501.5821.5551.5691.4741251.2481.1991.2241.12962.50.7090.6990.7040.60931.250.4720.4460.4590.36415.60.2900.2890.2900.19500.1040.0980.095 | ||||||
本试剂盒所含材料：
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A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CAT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CAT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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