首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠半胱氨酸天冬氨酸蛋白酶1(CASP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织培养上清液样本中的CASP1含量。CASP1即胱天蛋白酶1，其在炎症反应和细胞凋亡中扮演重要角色。研究机制方面，它可激活白细胞介素等促炎因子，引发炎症级联反应。对其深入研究有助于理解炎症性疾病发病机制，为相关疾病治疗提供新方向。试剂盒检测范围为6.25 pg/mL-400 pg/mL，研究人员可将其应用于炎症机制研究、药物干预效果评估或细胞模型中CASP1动态变化的监测，特别在神经免疫、感染性疾病模型构建及免疫调节剂开发等基础研究中具有重要价值本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Casp1 ELISA kit; Il1bc ELISA kit; Il1bceCaspase-1 ELISA kit; CASP-1 ELISA kit; EC 3.4.22.36 ELISA kit; Interleukin-1 beta convertase ELISA kit; IL-1BC ELISA kit; Interleukin-1 beta-converting enzyme ELISA kit; ICE ELISA kit; IL-1 beta-converting enzyme ELISA kit; p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10] ELISA kit
缩写：CASP1
Uniprot No.：P43527
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell lysates, cell culture supernates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:1 | Average % | 91 | ||
| Range % | 88-93 | |||
| 1:2 | Average % | 95 | ||
| Range % | 90-102 | |||
| 1:4 | Average % | 97 | ||
| Range % | 94-101 | |||
| 1:8 | Average % | 94 | ||
| Range % | 91-98 |
回收率：
| The recovery of rat CASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 86-94 | |||||
| EDTA plasma (n=4) | 99 | 93-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 4002.1892.3212.2552.047 2001.7291.7541.7421.534 1001.1511.1651.1580.950 500.7550.7480.7520.544 250.4560.4680.4620.254 12.50.3170.3250.3210.113 6.250.2770.2900.2840.076 00.2010.2150.208 | |||||||
货期：3-5 working days
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