| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HPXEL |
| WB Predicted band size | 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human ECH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ECH1抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：*ECH1 antibody-based detection of mitochondrial dysfunction in hepatocellular carcinoma*
**作者**：Li, X., Zhang, Y., & Wang, H.
**摘要**：本研究利用特异性ECH1抗体，通过免疫组化技术分析肝癌组织中ECH1蛋白的表达水平，发现其下调与线粒体脂肪酸氧化受损及患者预后不良显著相关。
2. **文献名称**：*Development of a novel monoclonal ECH1 antibody for metabolic syndrome research*
**作者**：Smith, J.R., et al.
**摘要**：报道了一种高灵敏度的ECH1单克隆抗体的制备与验证，该抗体通过Western blot和流式细胞术证实可特异性识别人类及小鼠组织中的ECH1蛋白，为代谢综合征的分子机制研究提供工具。
3. **文献名称**：*ECH1 as a potential biomarker in Alzheimer’s disease: Insights from antibody-mediated proteomic analysis*
**作者**：Chen, L., et al.
**摘要**：通过抗ECH1抗体进行脑脊液蛋白质组学分析，发现阿尔茨海默病患者ECH1表达异常，提示其可能参与神经炎症及脂质代谢紊乱的病理过程。
(注：以上文献为示例性内容，实际引用需查询PubMed、Google Scholar等数据库获取真实文献。)
ECH1 (Enoyl-CoA Hydratase 1) is an enzyme involved in fatty acid β-oxidation, catalyzing the hydration of 2-trans-enoyl-CoA to 3-hydroxyacyl-CoA in mitochondria. It plays a critical role in energy metabolism and cellular homeostasis. Dysregulation of ECH1 has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, making it a research target for understanding disease mechanisms.
ECH1 antibodies are essential tools for detecting and quantifying ECH1 protein expression in various experimental applications, including Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are typically developed using immunogenic peptides or recombinant ECH1 protein fragments to ensure specificity. Validation steps, such as knockout controls or siRNA knockdown, are crucial to confirm antibody reliability.
Research utilizing ECH1 antibodies has revealed its tissue-specific expression patterns, with high levels in energy-demanding organs like the liver, heart, and kidneys. In cancer studies, altered ECH1 expression has been linked to tumor progression, metastasis, and chemoresistance, suggesting its potential as a therapeutic or diagnostic biomarker. Additionally, ECH1 antibodies aid in studying mitochondrial dysfunction in metabolic syndromes, providing insights into lipid metabolism imbalances.
Overall, ECH1 antibodies serve as pivotal reagents for exploring the enzyme’s biological roles and its implications in human diseases, bridging molecular insights to clinical applications.
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