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产品描述：人滤泡树突状细胞分泌蛋白(C4orf7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4orf7含量。试剂盒检测范围为7.81 ng/mL-500 ng/mL，适用于免疫学基础研究、疾病模型中的蛋白动态监测以及相关分子通路的机制探索等科研场景，为深入解析该蛋白的生物学功能提供可靠技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C4orf7 ELISA Kit; FDC secreted protein ELISA Kit; FDC-SP ELISA Kit; FDCSP ELISA Kit; FDSCP_HUMAN ELISA Kit; Follicular dendritic cell secreted peptide ELISA Kit; Follicular dendritic cell secreted protein ELISA Kit
缩写：C4orf7
Uniprot No.：Q8NFU4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.81 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 84-91 | ||||||
| 1:8 | Average % | 109 | |||||
| Range % | 105-114 | ||||||
回收率：
| The recovery of human C4orf7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-93 | |||||
| EDTA plasma (n=4) | 106 | 103-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected5002.2212.0942.1582.0462501.3451.4051.3751.2631250.7420.7890.7660.65462.50.4130.4020.4080.29631.250.3050.3310.3180.20615.620.2170.2080.2130.1017.810.1820.1780.1800.06800.1110.1130.112 | |||||||
货期：3-5 working days
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