首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体片段4d(C4d)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C4d含量。C4d是经典补体激活途径的相关产物，可反映补体系统激活状况。研究机制上，其产生与C4的裂解有关，检测C4d水平能辅助判断补体激活程度，在免疫相关疾病，如移植排斥反应等的诊断和病情监测中有重要价值。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于体外研究场景，如探讨补体系统在心血管疾病、肾脏病理或免疫复合物相关疾病中的作用机制，也可用于评估实验动物模型或细胞实验中补体激活水平，为免疫相关基础研究提供精准定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：C4d
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4d in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 103 | |||||
| Range % | 94-108 | ||||||
| 1:200 | Average % | 98 | |||||
| Range % | 90-104 | ||||||
| 1:400 | Average % | 89 | |||||
| Range % | 82-93 | ||||||
| 1:800 | Average % | 95 | |||||
| Range % | 88-98 | ||||||
回收率：
| The recovery of human C4d spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 113 | 103-117 | |||||
| EDTA plasma (n=4) | 94 | 89-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected502.8152.7472.7812.678252.3052.2322.2692.16612.51.4991.5371.5181.4156.250.7330.7450.7390.6363.120.3990.4130.4060.3031.560.2380.2310.2350.1320.780.1550.1660.1610.05800.1010.1050.103 | |||||||
货期：3-5 working days
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