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产品描述：人C4b结合蛋白β链(C4BPB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的C4BPB含量。人C4b结合蛋白β链（C4BPB）是补体系统中的重要调节因子，由β链和α链组成，主要功能是结合C3b/C4b，抑制补体系统的过度激活，从而保护机体免受自身免疫损伤。研究机制涉及C4BPB与C3b/C4b的相互作用及其在补体激活途径中的调节作用。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研中对补体系统调控机制的探索。广泛应用于炎症性疾病、自身免疫病等补体相关机制研究，支持细胞模型中蛋白分泌水平检测或动物组织样本分析，为免疫调控研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C4b binding protein beta chain ELISA Kit; C4b-binding protein beta chain ELISA Kit; C4BP ELISA Kit; C4BPB ELISA Kit; C4BPB_HUMAN ELISA Kit; Complement component 4 binding protein beta ELISA Kit; Complement component 4 binding protein beta chain ELISA Kit; Complement component 4-binding protein beta ELISA Kit; OTTHUMP00000034288 ELISA Kit; OTTHUMP00000034289 ELISA Kit; OTTHUMP00000034375 ELISA Kit; OTTHUMP0000003474 ELISA Kit; Proline rich protein ELISA Kit; PRP ELISA Kit
缩写：C4BPB
Uniprot No.：P20851
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4BPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 91-108 | |||||
| 1:2000 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:4000 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8000 | Average % | 97 | ||||
| Range % | 91-112 | |||||
回收率：
| The recovery of human C4BPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-105 | ||||
| EDTA plasma (n=4) | 95 | 89-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.9052.8752.8902.7551002.4892.4732.4812.346501.7341.7501.7421.607250.9480.9570.9530.81812.50.5120.5060.5090.3746.250.4640.4710.4680.3333.1250.2300.2340.2320.09700.1330.1360.135 | ||||||
货期：3-5 working days
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