首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体成分C1s(C1s)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1S含量。C1S是一种重要靶点。它参与补体系统激活，在免疫防御等方面有重要作用。研究机制方面聚焦于其结构与功能关系，探索调控其活性途径以开发治疗方法，有望用于免疫相关疾病如自身免疫病的干预。试剂盒检测范围为25 ng/mL-1600 ng/mL，适用于基础科研领域研究补体系统功能、探索自身免疫性疾病（如系统性红斑狼疮）、感染性疾病或神经退行性病变中的补体活化机制，同时可为药物开发中补体通路调控效果的体外评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Basic proline rich peptide IB 1 ELISA Kit; C1 esterase ELISA Kit; C1S ELISA Kit; C1S_HUMAN ELISA Kit; Complement C1s subcomponent ELISA Kit; Complement C1s subcomponent heavy chain ELISA Kit; Complement C1s subcomponent light chain ELISA Kit; Complement component 1 s subcomponent ELISA Kit; Complement component 1 subcomponent s ELISA Kit; FLJ44757 ELISA Kit
缩写：C1S
Uniprot No.：P09871
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 ng/mL-1600 ng/mL
灵敏度：6.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 100 | |||||
| Range % | 91-104 | ||||||
| 1:200 | Average % | 107 | |||||
| Range % | 98-110 | ||||||
| 1:400 | Average % | 88 | |||||
| Range % | 82-93 | ||||||
| 1:800 | Average % | 96 | |||||
| Range % | 87-99 | ||||||
回收率：
| The recovery of human C1S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 110 | 98-114 | |||||
| EDTA plasma (n=4) | 98 | 90-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 16002.7402.6852.7132.598 8002.3462.2762.3112.196 4001.4881.4541.4711.356 2000.7560.7680.7620.647 1000.3960.4110.4040.289 500.2460.2390.2430.128 250.1620.1700.1660.051 00.1130.1170.115 | |||||||
货期：3-5 working days
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