| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NU; HC2; PROS30; HEL-S-275 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PSMA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PSMA1(蛋白酶体亚基α1)抗体的参考文献示例(文献为虚构，仅作格式参考)：
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1. **文献名称**: *PSMA1 Expression in Acute Myeloid Leukemia: Prognostic Implications*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫组化分析PSMA1在急性髓系白血病(AML)患者中的表达水平，发现其高表达与不良预后相关。PSMA1抗体(克隆号3C7)用于组织切片染色，证实其在肿瘤细胞核内富集，提示其可能作为潜在治疗靶点。
2. **文献名称**: *Proteasome Subunit Alpha 1 (PSMA1) Antibody Validation for Western Blot Analysis*
**作者**: Smith J, et al.
**摘要**: 文章系统验证了商业化PSMA1抗体(货号AB123)的特异性，通过siRNA敲低实验和质谱分析证实其识别目标蛋白，并优化了Western Blot实验条件，为蛋白酶体功能研究提供可靠工具。
3. **文献名称**: *PSMA1 Localization in Neurodegenerative Disease Models*
**作者**: Lee H, et al.
**摘要**: 利用PSMA1特异性抗体进行免疫荧光染色，发现阿尔茨海默病模型小鼠脑组织中蛋白酶体亚基的异常聚集，提示蛋白酶体功能障碍可能与病理蛋白沉积相关。
4. **文献名称**: *Role of PSMA1 in Cell Cycle Regulation*
**作者**: García-Morales P, et al.
**摘要**: 通过抗体介导的PSMA1抑制实验，证明其参与细胞周期G1/S期转换调控，并揭示其与p53蛋白的相互作用机制，为癌症治疗提供新视角。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“PSMA1 antibody”或“proteasome subunit alpha 1”为关键词检索最新文献。若需具体文献，请提供更详细的研究背景或应用场景。
PSMA1 (Proteasome 20S Subunit Alpha 1) is a core component of the 20S proteasome, a multi-subunit complex central to the ubiquitin-proteasome system responsible for regulated protein degradation. As part of the 19S-20S-11S proteasome assemblies, PSMA1 forms the outer α-ring of the 20S catalytic core, facilitating substrate recognition, gate opening, and structural stability. Its role in maintaining cellular proteostasis impacts critical processes like cell cycle progression, stress response, and antigen presentation.
PSMA1 antibodies are essential tools for studying proteasome composition, dynamics, and dysfunction. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to detect PSMA1 expression, localization, and interaction partners. Given the proteasome's involvement in diseases like cancer and neurodegenerative disorders, PSMA1 antibodies help investigate pathological mechanisms, particularly in contexts of proteasome inhibition or overexpression. For instance, elevated PSMA1 levels in certain cancers correlate with drug resistance, making it a potential therapeutic biomarker.
Validated PSMA1 antibodies typically show specificity for the ~30 kDa protein across human and model organisms. Researchers prioritize antibodies verified in knockout controls to avoid cross-reactivity with other α-subunits (PSMA2-7). Commercial variants include monoclonal and polyclonal formats, often conjugated to fluorophores or enzymes for multiplex assays. Recent studies also utilize PSMA1 antibodies in proteasome activity profiling and single-particle cryo-EM structural analyses, underscoring their versatility in both basic and translational research.
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