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产品描述：人补体C1q肿瘤坏死因子相关蛋白6(C1QTNF6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1QTNF6含量。C1QTNF6是一种与炎症和肿瘤相关的蛋白，其高表达与多种癌症的预后不良相关。研究显示，C1QTNF6可能通过影响细胞迁移和侵袭能力来促进肿瘤发展，其具体机制涉及细胞周期、G2M检测点、上皮-间质转化和血管生成信号通路。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可将其应用于代谢性疾病机制研究、慢性炎症相关信号通路探索，或肿瘤微环境中细胞因子调控等领域，为揭示C1QTNF6在疾病发生发展中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1q and tumor necrosis factor related protein 6 ELISA Kit; C1QT6_HUMAN ELISA Kit; C1qtnf6 ELISA Kit; Complement C1q tumor necrosis factor related protein 6 ELISA Kit; Complement C1q tumor necrosis factor-related protein 6 ELISA Kit; CTRP 6 ELISA Kit; CTRP6 ELISA Kit; OTTHUMP00000197772 ELISA Kit; OTTHUMP00000197774 ELISA Kit; PRO1151 ELISA Kit; UNQ581 ELISA Kit; ZACRP6 ELISA Kit
缩写：C1QTNF6
Uniprot No.：Q9BXI9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 105 | ||||
| Range % | 99-108 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 84-94 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human C1QTNF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-96 | ||||
| EDTA plasma (n=4) | 90 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7782.6622.7202.600101.9261.9051.9161.79651.3151.3421.3291.2092.50.7030.7340.7190.5991.250.3900.3810.3860.2660.6250.2950.2880.2920.1720.3120.2030.2090.2060.08600.1190.1210.120 | ||||||
货期：3-5 working days
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