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产品描述：人C1q/TNF相关蛋白3(Cartonectin/CTRP3/CORS-26)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的C1QTNF3含量。C1q/TNF相关蛋白3（CTRP3）是一种分泌蛋白，属于CTRP家族，具有多种生理功能。研究发现，CTRP3在代谢、炎症和生存信号传导等方面发挥重要作用，与肥胖、2型糖尿病、心血管疾病等代谢相关疾病密切相关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于细胞生物学、基础医学等科研领域，可用于探究CTRP3在代谢性疾病模型中的表达调控机制、炎症相关信号通路研究，或评估药物干预对靶蛋白分泌水平的影响，为相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1ATNF3 ELISA Kit; C1q and tumor necrosis factor related protein 3 ELISA Kit; C1QT3_HUMAN ELISA Kit; C1qtnf3 ELISA Kit; Cartonectin ELISA Kit; Collagenous repeat containing sequence of 26 kDa ELISA Kit; collagenous repeat-containing sequence 26 kDa protein ELISA Kit; Complement C1q tumor necrosis factor related protein 3 ELISA Kit; Complement C1q tumor necrosis factor-related protein 3 ELISA Kit; Corcs ELISA Kit; CORS 26 ELISA Kit; Cors ELISA Kit; CORS26 ELISA Kit; CTRP3 ELISA Kit; FLJ37576 ELISA Kit; OTTHUMP00000115918 ELISA Kit; OTTHUMP00000219981 ELISA Kit; PRO1484 ELISA Kit; Secretory protein CORS26 ELISA Kit; UNQ753 ELISA Kit
缩写：C1QTNF3
Uniprot No.：Q9BXJ4
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CTRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-102 | |||||
回收率：
| The recovery of human CTRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 85-100 | ||||
| EDTA plasma (n=4) | 101 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected101.8391.9521.8961.74151.6161.5861.6011.4462.51.3541.2711.3131.1581.250.9120.9320.9220.7670.6250.6170.6090.6130.4580.3120.4120.3880.4000.2450.1560.2630.2510.2570.10200.1590.1510.155 | ||||||
货期：3-5 working days
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