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产品描述：人补体C1q肿瘤坏死因子相关蛋白2(C1QTNF2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1QTNF2含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研人员可通过探索C1QTNF2在肥胖、糖尿病、动脉粥样硬化等疾病模型中的表达变化，或用于评估药物干预对代谢相关信号通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1q and tumor necrosis factor related protein 2 ELISA Kit; C1QT2_HUMAN ELISA Kit; C1qtnf2 ELISA Kit; Complement C1q tumor necrosis factor-related protein 2 ELISA Kit; CTRP2 ELISA Kit; Zacrp2 ELISA Kit
缩写：C1QTNF2
Uniprot No.：Q9BXJ5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:10 | Average % | 103 | |||||
| Range % | 98-106 | ||||||
| 1:20 | Average % | 110 | |||||
| Range % | 106-114 | ||||||
| 1:40 | Average % | 92 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of human C1QTNF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 81-89 | |||||
| EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.5322.6042.5682.38252.0591.9872.0231.8372.51.3071.3371.3221.1361.250.7730.7680.7710.5850.6250.5030.5120.5080.3220.3120.3070.3140.3110.1250.1560.2470.2570.2520.06600.1850.1870.186 | |||||||
货期：3-5 working days
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