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产品描述：人补体C1q样蛋白3(C1QL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C1QL3含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，支持科研人员通过血清或血浆样本探索C1QL3在生理病理过程中的动态变化，或利用组织匀浆分析其在特定器官中的表达特征，为代谢调控机制、神经免疫互作等基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1q and tumor necrosis factor-related protein 13 ELISA Kit; C1q/TNF-related protein 13 ELISA Kit; C1ql ELISA Kit; C1QL3 ELISA Kit; C1QL3_HUMAN ELISA Kit; C1QTNF13 ELISA Kit; Complement C1q-like protein 3 ELISA Kit; Complement component 1; q subcomponent-like 3 ELISA Kit; CTRP13 ELISA Kit; K100 ELISA Kit
缩写：C1QL3
Uniprot No.：Q5VWW1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1QL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-107 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human C1QL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.5102.3502.4302.292 101.9611.8561.9091.771 51.2761.3241.3001.162 2.50.7990.8250.8120.674 1.250.4530.4270.4400.302 0.6250.3470.3720.3600.222 0.3120.2620.2590.2610.123 00.1410.1350.138 | ||||||
货期：3-5 working days
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