首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD147分子(CD147)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的BSG含量。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于基础科研中探索CD147在肿瘤生物学、感染性疾病或自身免疫性疾病中的功能机制；研究者可通过定量分析不同病理状态下血清或血浆中CD147的动态变化，为疾病模型构建、药物干预效果评估或生物标志物筛选提供可靠数据支持，适用于细胞实验、动物模型及临床前样本分析等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：5A11 antigen ELISA Kit; 5F7 ELISA Kit; BASI_HUMAN ELISA Kit; Basigin (Ok blood group) ELISA Kit; Basigin ELISA Kit; Blood brain barrier HT7 antigen ELISA Kit; Bsg ELISA Kit; CD 147 ELISA Kit; CD147 ELISA Kit; CD147 antigen ELISA Kit; Collagenase stimulatory factor ELISA Kit; EMMPRIN ELISA Kit; Extracellular matrix metalloproteinase inducer ELISA Kit; Leukocyte activation antigen M6 ELISA Kit; M 6 ELISA Kit; M6 ELISA Kit; M6 leukocyte activation antigen ELISA Kit; Neurothelin ELISA Kit; OK ELISA Kit; OK blood group ELISA Kit; OK blood group antigen ELISA Kit; TCSF ELISA Kit; Tumor cell derived collagenase stimulatory factor ELISA Kit; Tumor cell-derived collagenase stimulatory factor ELISA Kit
缩写：BSG
Uniprot No.：P35613
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD147 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 93-104 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 93-100 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of human CD147 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected8002.1122.2022.1572.0534001.7561.7351.7461.6422001.1421.2011.1721.0681000.7250.7410.7330.629500.4130.4050.4090.305250.2780.2810.2800.17612.50.2110.2000.2060.10200.1050.1030.104 | |||||||
货期：3-5 working days
×
