首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人杀菌性/通透性增加蛋白(BPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的BPI含量。BPI即杀菌通透性增加蛋白，它能与革兰阴性菌的脂多糖结合，发挥杀菌及中和内毒素等作用。研究机制方面，其抗菌作用主要通过破坏细菌外膜，还可调节免疫细胞功能，在抗感染、炎症调控等方面研究较多，有潜在临床应用价值。试剂盒检测范围为1.25 ng/mL-80 ng/mL，其标准化操作流程适用于体外实验研究，例如探索炎症反应中中性粒细胞功能、评估感染性疾病模型中宿主防御机制的变化，或监测药物干预对免疫调节蛋白表达的影响。该产品可为免疫学、微生物学及药理学领域的科研人员提供可靠工具，用于分析BPI在宿主 - 病原体相互作用中的动态变化，或作为实验性治疗方案的生物标志物研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bactericidal permeability-increasing protein ELISA Kit; Bactericidal/permeability increasing protein ELISA Kit; BPI ELISA Kit; BPI fold containing family D; member 1 ELISA Kit; BPI_HUMAN ELISA Kit; BPIFD1 ELISA Kit; CAP 57 ELISA Kit; CAP57 ELISA Kit; rBPI ELISA Kit; Recombinant BPI holoprotein; rBPI ELISA Kit
缩写：BPI
Uniprot No.：P17213
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human BPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected802.6952.7322.7142.618402.2852.1542.2202.124201.4311.3661.3991.303100.8270.8810.8540.75850.4120.4290.4210.3252.50.2580.2470.2530.1571.250.1690.1750.1720.07600.0940.0980.096 | |||||||
货期：3-5 working days
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