首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脑钠素/脑钠尿肽(BNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BNP含量。BNP即脑钠肽，由心室肌细胞合成分泌。当心室容量增加和压力负荷过重时其分泌增多。研究上，BNP可反映心室功能，是诊断心衰等疾病的重要生物标志物。通过检测其水平变化，能评估病情、判断预后及指导治疗。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于心血管疾病机制研究、心脏功能评估模型构建、药物干预效果评价等科研领域，为探索BNP在病理生理过程中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NppbNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide ELISA kit; Iso-ANP) [Cleaved into: Brain natriuretic peptide 45 ELISA kit; 5 kDa cardiac natriuretic peptide ELISA kit; BNP-34)] ELISA kit
缩写：BNP
Uniprot No.：P13205
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.8952.8982.8972.83540002.2942.3262.3102.24820001.3271.2921.3101.24810000.7070.6890.6980.6365000.3450.3170.3310.2692500.1960.1840.1900.1281250.1170.1140.1160.05400.0640.0600.062 | ||||||
货期：3-5 working days
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