首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脑钠素/脑钠尿肽(BNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆、组织培养上清液样本中的BNP含量。BNP（脑钠肽）是一种由心脏分泌的肽类激素，主要在心室壁受到扩张或牵拉时合成。其机制包括舒张血管、降低血压、利钠利尿和抑制交感神经等，对心血管系统具有调节作用。研究BNP有助于了解心衰的诊断、治疗和预后评估。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，适用于心血管疾病模型构建、药物作用机制研究及生物标志物筛查等科研领域，为心功能相关的基础研究提供高效分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ANFB_HUMAN ELISA Kit; BNP ELISA Kit; BNP(1-32) ELISA Kit; BNP(5-29) ELISA Kit; BNP-32 ELISA Kit; Brain type natriuretic peptide ELISA Kit; Gamma brain natriuretic peptide ELISA Kit; Gamma-brain natriuretic peptide ELISA Kit; Natriuretic peptide precursor B ELISA Kit; nppb ELISA Kit; NT proBNP ELISA Kit
缩写：BNP
Uniprot No.：P16860
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates, cell culture supernates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.43 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.3112.2562.2842.17930001.9051.8761.8911.78615001.5481.4471.4981.3937501.1021.0591.0810.9763750.8710.8570.8640.759187.50.5020.4900.4960.39193.750.3130.2970.3050.20000.1090.1010.105 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BNP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled BNP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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