首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨成型蛋白受体Ⅱ(BMPR-Ⅱ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BMPR2含量。BMPR2（骨形态发生蛋白受体II型）是骨形态发生蛋白（BMP）受体家族的一员，参与骨骼发育、细胞增殖和分化。其异常活动与多种疾病相关，尤其是肺动脉高压（PAH）的发病机制密切相关。研究显示，BMPR2突变可能导致肺血管平滑肌细胞异常增殖和肺血管重构，从而引起PAH。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品为研究BMPR - Ⅱ在细胞信号转导、组织再生调控等机制提供了可靠工具，可应用于肺高压模型、骨代谢研究、肿瘤微环境分析等科研场景中蛋白表达水平变化的动态监测，也可用于药物干预或基因编辑后生物标志物的定量评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP type II receptor ELISA Kit; BMP type-2 receptor ELISA Kit; BMPR 2 ELISA Kit; BMPR 3 ELISA Kit; BMPR II ELISA Kit; BMPR-2 ELISA Kit; BMPR-II ELISA Kit; Bmpr2 ELISA Kit; BMPR2_HUMAN ELISA Kit; BMPR3 ELISA Kit; BMPRII ELISA Kit; BMR 2 ELISA Kit; BMR2 ELISA Kit; Bone morphogenetic protein receptor type 2 ELISA Kit; Bone morphogenetic protein receptor type II ELISA Kit; Bone morphogenetic protein receptor type-2 ELISA Kit; Bone morphogenic protein receptor type II serine threonine kinase ELISA Kit; BRK 3 ELISA Kit; BRK3 ELISA Kit; PPH 1 ELISA Kit; PPH1 ELISA Kit; Serine threonine kinase type II activin receptor like kinase ELISA Kit; T ALK ELISA Kit; TALK ELISA Kit; Type II activin receptor like kinase ELISA Kit
缩写：BMPR2
Uniprot No.：Q13873
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMPR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human BMPR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-96 | ||||
| EDTA plasma (n=4) | 93 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.4922.4372.4652.3012002.0542.0762.0651.9011001.6711.6341.6531.489501.0741.0431.0590.895250.6710.6530.6620.49812.50.4830.4770.4800.3166.250.2710.2640.2680.10400.1620.1660.164 | ||||||
货期：3-5 working days
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