| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FAM31B; C1ORF18; C1orf218 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DENND1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DENND1B抗体的3篇参考文献示例(注：文献为假设性概括，实际文献需通过数据库查询确认)：
1. **标题**: *DENND1B Variants Regulate Th2 Cell-Mediated Airway Inflammation*
**作者**: Larose et al.
**摘要**: 研究发现了DENND1B基因多态性与儿童哮喘的关联，通过抗体检测发现DENND1B蛋白在T细胞中高表达，并调控Th2细胞因子的分泌，影响气道炎症反应。
2. **标题**: *DENND1B Functions as a Guanine Nucleotide Exchange Factor in Rab35 Signaling*
**作者**: McNamara et al.
**摘要**: 利用DENND1B特异性抗体进行免疫沉淀实验，揭示了DENND1B作为Rab35 GTP酶的激活因子，参与细胞膜运输和突触形成，为神经发育疾病机制提供依据。
3. **标题**: *DENND1B Expression in Autoimmune Thyroid Disease*
**作者**: O'Connell et al.
**摘要**: 通过Western blot和免疫组化分析，发现DENND1B抗体在甲状腺滤泡细胞中的异常表达与Graves病相关，提示其在自身免疫调控中的作用。
4. **标题**: *DENND1B Mutation Analysis in Pediatric Immune Dysregulation*
**作者**: Puffenberger et al.
**摘要**: 研究利用DENND1B抗体验证患者突变体的蛋白表达缺失，发现其与早发型免疫失调综合征相关，强调该基因在淋巴细胞功能中的关键性。
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**提示**：以上为模拟示例，真实文献请通过PubMed或Google Scholar搜索关键词“DENND1B antibody”或“DENND1B function”获取。
DENND1B (Differentially Expressed in Normal and Neoplastic Cells Domain 1B) is a member of the DENN domain-containing protein family, which plays critical roles in intracellular membrane trafficking and signal transduction. The DENND1B gene encodes a guanine nucleotide exchange factor (GEF) that activates Rab GTPases, particularly Rab35. regulating vesicle formation, receptor recycling, and cytokine secretion. DENND1B is implicated in immune regulation, neurodevelopment, and cancer progression, with studies linking its genetic variants to asthma, autoimmune diseases, and neurodevelopmental disorders.
DENND1B antibodies are essential tools for studying the protein's expression, localization, and function in cellular and disease contexts. These antibodies enable detection of DENND1B via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research using DENND1B-specific antibodies has revealed its involvement in T-cell receptor signaling, dendritic cell maturation, and synaptic vesicle trafficking. In cancer, DENND1B overexpression has been associated with tumor growth and metastasis in certain malignancies. The antibody's applications extend to validating CRISPR/Cas9 knockout models and exploring DENND1B-Rab35 pathway dysregulation in pathological conditions. Commercially available DENND1B antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown, ensuring reliability in experimental settings.
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