首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨形成蛋白6(BMP-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的BMP6含量。BMP6，即骨形态发生蛋白6，是TGF-β超家族成员，参与骨骼发育、血管生成、铁代谢等过程。其研究机制涉及调节VEGF信号通路、Hippo信号通路等，对心血管疾病、癌症等有潜在治疗价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于体外研究领域，如骨骼生物学、代谢调控机制探索、肿瘤微环境分析等科研场景，为研究者提供稳定可靠的BMP - 6蛋白定量工具，助力细胞信号通路、疾病模型构建及药物干预效果评估等实验方向的数据获取。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP-6 ELISA Kit; Bmp6 ELISA Kit; BMP6_HUMAN ELISA Kit; Bone morphogenetic protein 6 ELISA Kit; Bone Morphogenic Protein 6 ELISA Kit; Decapentaplegic vegetal related ELISA Kit; DVR6 ELISA Kit; HGNC:12686 ELISA Kit; TGFB related vegetal related growth factor ELISA Kit; Transforming growth factor beta ELISA Kit; Vegetal related (TGFB related) cytokine ELISA Kit; Vegetal related growth factor (TGFB related) ELISA Kit; Vg related sequence ELISA Kit; VG-1-R ELISA Kit; VG-1-related protein ELISA Kit; Vg1 related sequence ELISA Kit; VGR ELISA Kit; VGR-1 ELISA Kit; VGR1 ELISA Kit
缩写：BMP6
Uniprot No.：P22004
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BMP-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 84-105 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human BMP-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-101 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2802.2172.2492.15410001.9251.9871.9561.8615001.5151.6241.5701.4752501.0481.0081.0280.9331250.5960.5830.5900.49562.50.3740.3550.3650.27031.250.2410.2520.2470.15200.0960.0930.095 | ||||||
货期：3-5 working days
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