首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠骨成型蛋白4(BMP-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BMP4含量。BMP4，即骨形态发生蛋白4，是TGF-β超家族成员，具有促进成骨、血管生成、胚胎分化和神经干细胞分化等功能。其研究背景涉及心血管疾病、骨再生和肿瘤生长等，机制上通过调节细胞增殖、分化和迁移等过程发挥作用。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本试剂盒适用于科研领域中对BMP - 4功能机制的探索，例如骨骼发育动态研究、再生医学中组织修复模型的分子机制解析、胚胎发育过程中形态发生调控的定量分析，以及相关疾病模型的分子标志物检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
缩写：BMP4
Uniprot No.：Q06826
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.5142.6512.5832.4682501.8671.7971.8321.7171251.2791.3151.2971.18262.50.8850.8420.8640.74931.20.5130.5380.5260.41115.60.2940.2890.2920.1777.80.2010.2130.2070.09200.1120.1180.115 | ||||||
货期：3-5 working days
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