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产品描述:人杆状病毒IAP重复序列包含蛋白3(BIRC3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的BIRC3含量。BIRC3(Baculoviral IAP repeat-containing protein 3)是一种多功能的蛋白,参与细胞凋亡、炎症信号、免疫、细胞增殖、侵袭和转移的调节。其通过E3泛素连接酶活性调节NF-kappa-B信号通路,抑制非经典NF-kappa-B信号通路的构成激活。BIRC3在细胞凋亡过程中起重要作用,通过泛素化RIPK1和CASP8抑制ripoptosome的形成。试剂盒检测范围为37.5 pg/mL-2400 pg/mL,该产品适用于基础科研中细胞凋亡调控机制研究、肿瘤微环境分析以及抗癌药物开发过程中的生物标志物检测本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:AIP 1 ELISA Kit; AIP1 ELISA Kit; API 2 ELISA Kit; API2 ELISA Kit; Apoptosis inhibitor 2 ELISA Kit; Baculoviral IAP repeat containing 3 ELISA Kit; Baculoviral IAP repeat containing protein 3 ELISA Kit; Baculoviral IAP repeat-containing protein 3 ELISA Kit; BIRC 3 ELISA Kit; BIRC3 ELISA Kit; BIRC3_HUMAN ELISA Kit; C IAP2 ELISA Kit; C-IAP2 ELISA Kit; Cellular inhibitor of apoptosis 2 ELISA Kit; cellular inhibitor of apoptosis protein 2 ELISA Kit; CIAP 2 ELISA Kit; CIAP2 ELISA Kit; HAIP 1 ELISA Kit; HAIP1 ELISA Kit; HIAP 1 ELISA Kit; HIAP-1 ELISA Kit; HIAP1 ELISA Kit; IAP homolog C ELISA Kit; IAP-1 ELISA Kit; Inhibitor of apoptosis protein 1 ELISA Kit; MALT 2 ELISA Kit; MALT2 ELISA Kit; Mammalian IAP homolog C ELISA Kit; MIHC ELISA Kit; RING finger protein 49 ELISA Kit; RNF49 ELISA Kit; TNFR2 TRAF signaling complex protein 1 ELISA Kit; TNFR2 TRAF signalling complex protein ELISA Kit; TNFR2-TRAF-signaling complex protein 1 ELISA Kit
缩写:BIRC3
Uniprot No.:Q13489
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:37.5 pg/mL-2400 pg/mL
灵敏度:9.4 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human BIRC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 91 | |||||
Range % | 86-94 | ||||||
1:2 | Average % | 92 | |||||
Range % | 87-95 | ||||||
1:4 | Average % | 94 | |||||
Range % | 87-98 | ||||||
1:8 | Average % | 103 | |||||
Range % | 96-107 |
回收率:
The recovery of human BIRC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 91 | 86-94 | |||||
EDTA plasma (n=4) | 103 | 98-107 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected24002.1542.2742.2142.11212001.2681.3181.2931.1916000.7410.7980.7700.6683000.4430.4600.4520.3501500.2920.2850.2890.187750.1670.1740.1710.06937.50.1320.1390.1360.03400.1010.1020.102 |
货期:3-5 working days
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