首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人杆状病毒IAP重复序列包含蛋白2(BIRC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的BIRC2含量。BIRC2作为一个靶点，它属于杆状病毒IAP重复序列（BIRC）蛋白家族，在细胞凋亡调控中起到关键作用。研究发现其高表达与肿瘤的发生、发展密切相关。机制上，它主要通过抑制半胱天冬酶的活性来阻止细胞凋亡，是肿瘤治疗研究的重点方向。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于体外研究BIRC2在细胞凋亡机制中的功能、相关疾病模型的分子标志物分析，以及药物干预下蛋白表达变化的动态监测，为细胞信号转导研究、抗凋亡药物开发及病理机制探索提供标准化检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：API 1 ELISA Kit; API1 ELISA Kit; Apoptosis inhibitor 1 ELISA Kit; Baculoviral IAP repeat containing 2 ELISA Kit; Baculoviral IAP repeat containing protein 2 ELISA Kit; Baculoviral IAP repeat-containing protein 2 ELISA Kit; BIRC 2 ELISA Kit; BIRC2 ELISA Kit; BIRC2_HUMAN ELISA Kit; C IAP1 ELISA Kit; C-IAP1 ELISA Kit; Cellular inhibitor of apoptosis 1 ELISA Kit; cellular inhibitor of apoptosis protein 1 ELISA Kit; cIAP 1 ELISA Kit; cIAP1 ELISA Kit; HIAP 2 ELISA Kit; HIAP-2 ELISA Kit; HIAP2 ELISA Kit; IAP 2 ELISA Kit; IAP homolog B ELISA Kit; IAP-2 ELISA Kit; IAP2 ELISA Kit; Inhibitor of apoptosis protein 2 ELISA Kit; MIHB ELISA Kit; NFR2 TRAF signalling complex protein ELISA Kit; RING finger protein 48 ELISA Kit; RNF 48 ELISA Kit; RNF48 ELISA Kit; TNFR2 TRAF signaling complex protein 2 ELISA Kit; TNFR2-TRAF-signaling complex protein 2 ELISA Kit
缩写：BIRC2
Uniprot No.：Q13490
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BIRC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 897-98 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 96-107 | ||||||
回收率：
| The recovery of human BIRC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 82-94 | |||||
| EDTA plasma (n=4) | 101 | 97-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.0962.1132.1051.9477501.2781.3871.3331.1753750.8390.8670.8530.695187.50.5560.5150.5360.378940.3400.3480.3440.186470.2770.2870.2820.12423.50.2250.2310.2280.07000.1570.1580.158 | |||||||
货期：3-5 working days
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