首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠自噬蛋白(BECN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BECN1含量。BECN1是重要的自噬相关靶点。其参与自噬起始调控，在细胞稳态维持中发挥关键作用。当下研究聚焦于其在疾病发生发展中的机制，如肿瘤的生长、转移等，有望通过调控BECN1为相关疾病治疗提供新策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于研究自噬相关疾病模型中BECN1的蛋白表达动态，如阿尔茨海默病、帕金森病等神经系统疾病，或癌症、代谢综合征等实验动物模型的机制探索。满足基础科研中对自噬调控机制、药物干预效果评估等研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Becn1Beclin-1 ELISA kit; Coiled-coil myosin-like BCL2-interacting protein ELISA kit; Protein GT197) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa] ELISA kit
缩写：BECN1
Uniprot No.：Q91XJ1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BECN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-90 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of rat BECN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-98 | ||||
| EDTA plasma (n=4) | 90 | 86-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.1231.9822.0531.964 51.5391.5231.5311.442 2.51.0171.0011.0090.920 1.250.6910.6870.6890.600 0.6250.4220.4120.4170.328 0.3120.2680.2590.2640.175 0.1560.1660.1610.1640.075 00.0910.0870.089 | ||||||
货期：3-5 working days
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