首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脑源性神经营养因子(BDNF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BDNF含量。BDNF即脑源性神经营养因子，是神经营养素家族成员。其背景上，它在神经系统发育、维持神经元存活及功能方面至关重要。研究机制显示，BDNF通过与特定受体结合，激活细胞内信号通路，促进神经元生长、分化，在学习、记忆等认知过程中发挥作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，支持科研人员在神经生物学、行为学或疾病模型中系统性探究BDNF的动态表达规律，例如通过分析脑组织匀浆的BDNF水平评估外界刺激或药物干预对神经功能的影响，或结合血浆样本研究外周与中枢神经系统的关联机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BdnfBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
缩写：BDNF
Uniprot No.：P23363
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 105 | ||||
| Range % | 100-110 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-104 | |||||
回收率：
| The recovery of rat BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 98 | 93-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.2562.2992.2782.164102.0212.0132.0171.90351.6571.6741.6661.5522.51.2431.2671.2551.1411.250.7510.7640.7580.6440.6250.5020.5290.5160.4020.3120.3120.3210.3170.20300.1130.1150.114 | ||||||
货期：3-5 working days
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