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产品描述：人Bcl-2样蛋白1(BCL2L1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BCL2L1含量。BCL2L1是重要的靶点。它在细胞凋亡调节中起关键作用，异常表达与多种肿瘤发生发展相关。研究主要围绕其调控细胞存活信号、与凋亡蛋白相互作用机制等展开，旨在开发针对性药物以阻断其功能，实现肿瘤治疗。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础科研中探索疾病模型、药物干预或基因调控下BCL2L1的动态表达；广泛应用于细胞凋亡机制研究、抗肿瘤药物筛选、心血管疾病模型分析等领域，特别适用于比较不同组织或体液样本中BCL2L1的浓度差异，为疾病相关信号通路研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoptosis regulator Bcl X ELISA Kit; Apoptosis regulator Bcl-X ELISA Kit; Apoptosis regulator BclX ELISA Kit; B cell lymphoma 2 like ELISA Kit; B2CL1_HUMAN ELISA Kit; Bcl 2 like 1 protein ELISA Kit; Bcl X ELISA Kit; Bcl xL ELISA Kit; BCL XL/S ELISA Kit; Bcl xS ELISA Kit; Bcl-2-like protein 1 ELISA Kit; Bcl2 Like 1 ELISA Kit; Bcl2 related gene ELISA Kit; Bcl2-L-1 ELISA Kit; BCL2L ELISA Kit; Bcl2l1 ELISA Kit; BCLX ELISA Kit; BclXL ELISA Kit; BclXs ELISA Kit; DKFZp781P2092 ELISA Kit; PPP1R52 ELISA Kit; Protein phosphatase 1 regulatory subunit 52 ELISA Kit
缩写：BCL2L1
Uniprot No.：Q07817
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BCL2L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 88-95 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 94-103 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of human BCL2L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-94 | |||||
| EDTA plasma (n=4) | 96 | 94-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected402.6132.5452.5792.396201.9471.8551.9011.718101.1551.2241.1901.00750.7230.6840.7040.5212.50.4580.4400.4490.2661.250.3020.2920.2970.1140.6250.2330.2410.2370.05400.1880.1770.183 | |||||||
货期：3-5 working days
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