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Human bone alkaline phosphatase,BALP ELISA Kit

  • 中文名： 人骨碱性磷酸酶(BALP)ELISA Kit
货号: IPD-E09033h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人骨碱性磷酸酶(BALP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BALP含量。BALP即骨碱性磷酸酶，是成骨细胞的表型标志物之一。其背景在于它与骨代谢密切相关。研究机制上，主要通过检测其在血液中的活性水平，来反映成骨细胞的活性和功能，辅助评估骨骼生长、骨转换等情况，在骨相关疾病诊断等方面有重要价值。试剂盒检测范围为31.25 ng/ml-2000 ng/ml.，该产品广泛用于骨生物学研究、骨代谢相关药物开发评估、疾病动物模型骨形成能力分析等科研场景，为探究骨骼生长发育、病理机制及药物干预效果提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AKP2 ELISA Kit; Alkaline phosphatase liver/bone/kidney ELISA Kit; Alkaline phosphatase liver/bone/kidney isozyme ELISA Kit; Alkaline phosphatase tissue nonspecific isozyme ELISA Kit; Alkaline phosphatase, tissue-nonspecific isozyme ELISA Kit; Alkaline phosphomonoesterase ELISA Kit; Alpl ELISA Kit; AP TNAP ELISA Kit; AP-TNAP ELISA Kit; APTNAP ELISA Kit; BAP ELISA Kit; FLJ40094 ELISA Kit; FLJ93059 ELISA Kit; Glycerophosphatase ELISA Kit; HOPS ELISA Kit; Liver/bone/kidney type alkaline phosphatase ELISA Kit; MGC161443 ELISA Kit; MGC167935 ELISA Kit; PHOA ELISA Kit; PPBT_HUMAN ELISA Kit; Tissue non specific alkaline phosphatase ELISA Kit; Tissue nonspecific ALP ELISA Kit; TNAP ELISA Kit; TNSALP ELISA Kit
  • 缩写：
    BALP
  • Uniprot No.：
    P05186
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 ng/ml-2000 ng/ml.
  • 灵敏度：
    7.8 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    82-92


    1:2

    Average %

    97


    Range %

    91-104


    1:4

    Average %

    96


    Range %

    92-100


    1:8

    Average %

    95


    Range %

    90-100


  • 回收率：

    The recovery of human BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    89-96


    EDTA plasma (n=4)

    100

    96-104


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.458

    2.604

    2.531

    2.384


    1000

    1.688

    1.667

    1.677

    1.530


    500

    1.052

    1.028

    1.040

    0.893


    250

    0.697

    0.679

    0.688

    0.541


    125

    0.423

    0.411

    0.417

    0.270


    62.5

    0.318

    0.323

    0.321

    0.173


    31.25

    0.250

    0.244

    0.247

    0.100


    0

    0.150

    0.145

    0.147



  • 本试剂盒所含材料：

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    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human BALP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled BALP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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