首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β位淀粉样前体蛋白裂解酶1（BACE1）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BACE1含量。BACE1即β-分泌酶1，是阿尔茨海默病（AD）治疗的关键靶点。研究机制方面，它能切割淀粉样前体蛋白（APP）产生β-淀粉样蛋白（Aβ），Aβ异常聚集形成的斑块是AD典型病理特征，抑制BACE1可减少Aβ生成，为AD治疗带来希望。试剂盒检测范围为3.12 pg/mL-200 pg/mL，采用高特异性配对抗体实现精准检测，适用于疾病机制探索、药物靶点验证及病理模型分析等科研领域，可兼容多种生物样本类型，为体外实验或动物模型中的BACE1表达水平动态监测提供可靠工具。其宽泛的检测范围满足低丰度样本分析需求，尤其适用于神经科学、分子药理学等基础研究中对BACE1活性调控或抑制剂筛选的功能性研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BACE1; BACE; KIAA1149; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2
缩写：BACE1
Uniprot No.：P56817
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BACE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 82-97 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-105 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 85-101 | |||||
回收率：
| The recovery of human BACE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 80-100 | ||||
| EDTA plasma (n=4) | 98 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2002.1682.0522.1102.017 1001.9071.8601.8841.791 501.5951.4941.5451.452 251.3241.2391.2821.189 12.50.9060.8740.8900.797 6.250.4290.4090.4190.326 3.120.2770.2690.2730.180 00.0960.0900.093 | ||||||
货期：3-5 working days
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