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产品描述:人V型质子ATP酶116kDa亚基α亚型3(ATP6V0A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ATP6V0A2含量。ATP6V0A2是重要靶点。它参与细胞内pH调节等生理过程。相关研究聚焦其在疾病发生发展中的作用机制,如在肿瘤细胞增殖、转移等方面可能扮演关键角色,探索针对该靶点的干预措施有望为疾病治疗带来新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,适用于科研领域探索该蛋白在疾病模型中的表达变化、调控机制及其与细胞酸化功能关联的研究;适用于基础医学研究、分子病理学分析及生物标志物筛选等非诊断用途的科研场景本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:a2 ELISA Kit; A2V ATPase ELISA Kit; ARCL ELISA Kit; ATP6a2 ELISA Kit; ATP6N1D ELISA Kit; ATP6V0A2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a isoform 2 ELISA Kit; ATPase, H+ transporting, lysosomal V0 subunit a2 ELISA Kit; Infantile malignant osteopetrosis ELISA Kit; J6B7 ELISA Kit; Lysosomal H(+) transporting ATPase V0 subunit a2 ELISA Kit; Lysosomal H(+)-transporting ATPase V0 subunit a2 ELISA Kit; regeneration and tolerance factor ELISA Kit; Stv1 ELISA Kit; TJ6 ELISA Kit; TJ6M ELISA Kit; TJ6s ELISA Kit; V ATPase 116 kDa isoform a2 ELISA Kit; V type proton ATPase 116 kDa subunit a ELISA Kit; V type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; V-ATPase 116 kDa isoform a2 ELISA Kit; V-type proton ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a ELISA Kit; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2 ELISA Kit; Vph1 ELISA Kit; VPP2_HUMAN ELISA Kit; WSS ELISA Kit
缩写:ATP6V0A2
Uniprot No.:Q9Y487
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:31.25 pg/mL-2000 pg/mL
灵敏度:7.81 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ATP6V0A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 102 | ||||
Range % | 97-107 | |||||
1:4 | Average % | 91 | ||||
Range % | 85-97 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-103 |
回收率:
The recovery of human ATP6V0A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected 20002.2812.2982.2902.098 10001.6151.6421.6291.437 5000.9950.9540.9750.783 2500.6340.6260.6300.438 1250.4680.4490.4590.267 62.50.3580.3370.3480.156 31.250.2870.2980.2930.101 00.1910.1930.192 |
货期:3-5 working days
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