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产品描述：人ATP合成酶脂质结合蛋白，线粒体(ATP5G1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ATP5G1含量。ATP5G1是线粒体ATP合成酶的一个亚基，参与细胞能量代谢。其研究机制涉及线粒体ATP合成酶的组装和功能，通过氧化磷酸化过程合成ATP，为细胞提供能量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于基础科研领域对能量代谢机制的研究，可用于探索细胞代谢异常、神经退行性疾病模型中线粒体功能障碍、心血管疾病能量代谢调控，以及衰老相关线粒体活性变化等课题。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATP5MC1; ATP5G1; ATP synthase F(0 complex subunit C1, mitochondrial; ATP synthase lipid-binding protein; ATP synthase membrane subunit c locus 1; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F(0 complex subunit C1; ATPase protein 9; ATPase subunit c
缩写：ATP5G1
Uniprot No.：P05496
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-97 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.6372.6122.6252.4807502.2152.1182.1672.0223751.6971.6831.6901.545187.51.1071.1181.1130.968940.6110.6210.6160.471470.3650.3720.3690.22423.50.2420.2310.2370.09200.1460.1440.145 | ||||||
货期：3-5 working days
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