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产品描述：人ATP合成酶F1亚基β，线粒体(ATP5B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ATP5B含量。ATP5B是线粒体ATP合酶的β亚基，参与细胞能量代谢。其研究机制涉及原核表达和单克隆抗体制备，对肿瘤发生发展有重要作用。ATP5B表达异常与多种肿瘤相关，如结直肠癌、恶性胶质瘤等，成为潜在治疗靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于研究线粒体功能调控、能量代谢相关信号通路，以及疾病模型中ATP5B的动态变化，为体外实验提供高效稳定的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATP 5B ELISA Kit; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide ELISA Kit; ATP synthase subunit beta mitochondrial ELISA Kit; ATP synthase subunit beta; mitochondrial ELISA Kit; atp5b ELISA Kit; ATPB ELISA Kit; ATPB_HUMAN ELISA Kit; ATPMB ELISA Kit; ATPSB ELISA Kit; Epididymis secretory protein Li 271 ELISA Kit; HEL-S-271 ELISA Kit; Mitochondrial ATP synthase beta subunit ELISA Kit; Mitochondrial ATP Synthase Subunit Beta ELISA Kit; Mitochondrial ATP synthetase beta subunit ELISA Kit
缩写：ATP5B
Uniprot No.：P06576
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-91 | |||||
回收率：
| The recovery of human ATP5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.3082.3562.3322.1918001.5971.5581.5781.4374000.9190.9380.9290.7882000.5490.5870.5680.4271000.4050.4340.4200.279500.2930.2720.2830.142250.2010.2050.2030.06200.1410.1400.141 | ||||||
货期：3-5 working days
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