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产品描述：大鼠钠钾转运ATP酶亚基α-1(ATP1A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ATP1A1含量。ATP1A1是一种钠钾ATP酶的α1亚基，参与细胞内钠钾离子平衡和信号转导。其在肿瘤细胞中表达具有差异性，高表达与肿瘤增殖和患者生存期相关。研究机制涉及Src介导的信号转导和下游信号通路激活。试剂盒检测范围为31.2 pg/mL-2000 pg/mL，适用于基础科研领域，包括细胞离子转运机制研究、心血管或神经系统疾病模型中ATP酶功能异常的分子机制探索，以及药物干预对钠钾泵活性影响的体外评估，为相关领域提供精准的蛋白定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Atp1a1Sodium/potassium-transporting ATPase subunit alpha-1 ELISA kit; Na(+)/K(+) ATPase alpha-1 subunit ELISA kit; EC 7.2.2.13 ELISA kit; Sodium pump subunit alpha-1 ELISA kit
缩写：ATP1A1
Uniprot No.：P06685
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.2 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 83-90 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
回收率：
| The recovery of rat ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 81-89 | |||||
| EDTA plasma (n=4) | 98 | 94-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.6232.6012.6122.46410001.6861.7221.7041.5565001.1351.2021.1691.0212500.7590.7620.7610.6131250.5070.5130.5100.36262.50.3600.3520.3560.20831.20.2730.2680.2710.12300.1490.1470.148 | |||||||
货期：3-5 working days
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