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Human Ubiquitin-like modifier-activating enzyme ATG7(ATG7) ELISA kit

  • 中文名： 人ATG7自噬相关7同源物(s酵母) (ATG7)ELISA kit
货号: IPD-EL002294HU
Price： ￥2500
数量：
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  • 产品描述：人ATG7自噬相关7同源物(s酵母) (ATG7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ATG7含量。ATG7是一种自噬相关基因，其编码的蛋白质对自噬体形成和成熟至关重要。研究显示，ATG7敲除小鼠出生后不久就会死亡，表明其生存所必需。近期研究发现，ATG7缺失与人类复杂神经发育障碍有关，其缺失导致自噬通路异常，但患者仍能存活，提示人体具有补偿自噬功能的能力。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于探索自噬相关疾病模型（如神经退行性疾病、肿瘤等）中ATG7的表达调控，或评估药物干预、基因编辑等实验手段对自噬通路的影响。科研人员可通过该产品系统性分析ATG7在不同生理病理条件下的动态变化，为细胞生物学、分子机制研究及靶向药物开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：1810013K23Rik ELISA Kit; Apg 7 ELISA Kit; APG7 autophagy 7 like ELISA Kit; APG7 autophagy 7-like (S. cerevisiae) ELISA Kit; APG7 like ELISA Kit; APG7; S. cerevisiae; homolog of ELISA Kit; APG7-like ELISA Kit; APG7L ELISA Kit; ATG 7 ELISA Kit; ATG12-activating enzyme E1 ATG7 ELISA Kit; ATG7 ELISA Kit; ATG7 autophagy related 7 homolog (S. cerevisiae) ELISA Kit; ATG7 autophagy related 7 homolog ELISA Kit; ATG7_HUMAN ELISA Kit; Atg7l ELISA Kit; Autophagy 7; S. cerevisiae; homolog of ELISA Kit; Autophagy related protein 7 ELISA Kit; Autophagy-related 7 (yeast) ELISA Kit; Autophagy-related protein 7 ELISA Kit; DKFZp434N0735 ELISA Kit; GSA 7 ELISA Kit; GSA7 ELISA Kit; hAGP7 ELISA Kit; Ubiquitin activating enzyme E1 like protein ELISA Kit; Ubiquitin-activating enzyme E1-like protein ELISA Kit; Ubiquitin-like modifier-activating enzyme ATG7 ELISA Kit

  • 缩写：ATG7

  • Uniprot No.：O95352

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates, cell lysates

  • 检测范围：28 pg/mL-1800 pg/mL

  • 灵敏度：7 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Cell Biology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ATG7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %86

    Range %82-90

    1:2Average %101

    Range %97-104

    1:4Average %98

    Range %94-102

    1:8Average %105

    Range %101-109

  • 回收率：

    The recovery of human ATG7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)8481-89

    EDTA plasma (n=4)10197-104

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected18002.5392.6392.5892.4669002.0582.0042.0311.9084501.2751.3681.3221.1992250.7010.7240.7130.590112.50.4010.3940.3980.275560.2530.2480.2510.128280.1900.1870.1890.06600.1240.1220.123　







  • 货期：3-5 working days

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