首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抗凝血酶III(AT III)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AT III含量。AT III（抗凝血酶III）是一种由肝脏和血管内皮细胞合成的丝氨酸蛋白酶抑制蛋白，是人体内重要的抗凝血因子，在生理性凝血机制中起关键作用。其研究机制主要涉及通过抑制凝血酶及多种凝血因子活性来防止血栓形成，临床应用中主要用于治疗血栓栓塞性疾病。试剂盒检测范围为39 ng/mL-2500 ng/mL，适用于各类科研实验中AT III水平的精确分析，尤其适用于心血管疾病模型、药物抗凝机制研究、肝脏功能评估等科研场景，为探索凝血 - 抗凝系统调控及病理生理机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：AT III
Uniprot No.：Q5M7T5
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：39 ng/mL-2500 ng/mL
灵敏度：9.75 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:200 | Average % | 104 | |||||
| Range % | 100-112 | ||||||
| 1:400 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:1600 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 93 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 25002.4662.3122.3892.251 12502.0751.9962.0361.898 6251.6641.6891.6771.539 312.51.2381.2271.2331.095 1560.7020.7130.7080.570 780.4120.3920.4020.264 390.3010.2890.2950.157 00.1350.1410.138 | |||||||
货期：3-5 working days
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