首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人精氨基琥珀酸合成酶1(ASS1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ASS1含量。ASS1是精氨琥珀酸合成酶1，在尿素循环中催化瓜氨酸和天冬氨酸合成精氨琥珀酸。它可作为癌症治疗靶点，肿瘤细胞ASS1表达降低会依赖外源性精氨酸生长。研究聚焦调控其表达机制、探寻增强其活性药物，以开发新抗癌策略。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于研究ASS1在肝病、肿瘤代谢重编程或先天性代谢缺陷中的表达变化，或作为评估实验模型中尿素循环功能的工具；适用于细胞实验、动物模型或临床前研究中的机制探索，为代谢性疾病、癌症生物学或药物干预研究提供定量分析支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Argininosuccinate synthase 1 ELISA Kit; Argininosuccinate synthase ELISA Kit; Argininosuccinate synthetase 1 ELISA Kit; ASS ELISA Kit; Ass-1 ELISA Kit; ass1 ELISA Kit; ASSA ELISA Kit; ASSY_HUMAN ELISA Kit; Citrulline aspartate ligase ELISA Kit; Citrulline--aspartate ligase ELISA Kit; CTLN1 ELISA Kit
缩写：ASS1
Uniprot No.：P00966
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ASS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of human ASS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.9123.0212.9672.8312502.3802.5002.4402.3041251.6891.6751.6821.54662.50.9640.8890.9270.79131.20.5810.6030.5920.45615.60.4280.4120.4200.2847.80.2760.2780.2770.14100.1360.1350.136 | ||||||
货期：3-5 working days
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