首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人无孢蛋白(ASPN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ASPN含量。试剂盒检测范围为34.37 pg/mL-2200 pg/mL，本试剂盒适用于体外细胞模型、动物组织或体液样本的ASPN表达水平分析，为研究其在骨代谢疾病、纤维化病理机制或肿瘤微环境调控中的功能提供可靠工具，广泛应用于基础科研、药物靶点验证及生物标志物筛选等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ASPN ELISA Kit; ASPN protein ELISA Kit; ASPN_HUMAN ELISA Kit; Asporin (LRR class 1) ELISA Kit; Asporin ELISA Kit; Asporin proteoglycan ELISA Kit; FLJ20129 ELISA Kit; LRR class 1 ELISA Kit; Periodontal ligament associated protein 1 ELISA Kit; Periodontal ligament-associated protein 1 ELISA Kit; PLAP 1 ELISA Kit; PLAP-1 ELISA Kit; PLAP1 ELISA Kit; SLRR 1C ELISA Kit; SLRR1C ELISA Kit; Small leucine rich protein 1C ELISA Kit
缩写：ASPN
Uniprot No.：Q9BXN1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：34.37 pg/mL-2200 pg/mL
灵敏度：8.59 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ASPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 93-108 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 89-101 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 90-103 | ||||||
回收率：
| The recovery of human ASPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 94-104 | |||||
| EDTA plasma (n=4) | 92 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 22002.0592.0002.0301.916 11001.4511.3781.4151.301 5500.9290.9530.9410.827 2750.6590.6500.6550.541 137.50.3390.3440.3420.228 68.750.2610.2490.2550.141 34.370.1820.1780.1800.066 00.1160.1120.114 | |||||||
货期：3-5 working days
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