首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人促酰化蛋白(ASP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ASP含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探索肥胖症、2型糖尿病、代谢综合征等疾病的分子机制研究，也可用于体外实验模型中脂肪代谢相关药物或营养干预的效果评估，为代谢领域基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：ASP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ASP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:800 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human ASP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 100-110 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5272.4522.4902.39051.8581.9761.9171.8172.51.2041.2941.2491.1491.250.6570.6160.6370.5370.6250.3560.3770.3670.2670.3120.2010.2110.2060.1060.1560.1340.1290.1320.03200.0980.1010.100 | ||||||
货期：3-5 working days
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